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KLC & Tissue Culture Industries

January 13, 2021



Plant tissue culture is the main way of breeding seedlings in modern agriculture. In order to realize the industrialization of tissue culture, many plant factories have equipped their own seedling tissue culture workshops. However, most plant factories still have the problems of low yield, low quality and high production cost of tissue culture seedlings. The main reason is because of the high pollution.


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Generally speaking, in the tissue culture production process of plant factories, the pollution rate should be controlled within 5%. However, in actual production, it is very common that the pollution rate exceeds 5%, and some factories even exceed 10%. The higher the pollution rate, the higher the rejection rate of the tissue culture seedlings produced, which leads to a decrease in the quantity and quality of finished tissue culture seedlings, and the production cost of the factory also rises. So, where does the pollution come from?


ONE  Plant tissue culture and clean air


Before that, we first need to understand what plant tissue culture is.

Plant tissue culture refers to the cutting of organs, tissues or cells from plants. Aseptic culture is carried out on the artificially configured culture medium, and at the same time, by controlling the composition of the culture medium and other environmental factors such as temperature and light, the culture is directed to grow and differentiate to the process of developing and forming a complete plant. If the pollution is not well controlled during the period, it is easy to cause microbial infections such as bacteria/fungi in the culture, unable to grow and develop normally, and even die.


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According to research findings, most of the pollution sources (fungi/bacteria) appearing in plant tissue culture are derived from dust particles in the air, except for the bacteria carried by the explants and the incomplete sterilization of the medium/inoculation tools.


① Inoculation operation

During the inoculation operation, if the cleanliness of the air in the tissue culture room is not up to the standard, or the air filtration of the ultra-clean workbench is not thorough, these dust particles and microorganisms floating in the air will land when the operator comes into contact with the plants. It multiplies on plant tissues and culture media, causing tissue culture seedling pollution.


② Nurturing process

During the cultivation process of tissue culture seedlings, if the cleanliness of the cultivation room is poor, the airflow is fast, causing dust particles to move around, or the personnel entering the cultivation room have not been thoroughly sprayed and brought more mites and dust on their bodies, which will also cause the Cultivation pollution.


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—— Contaminated tissue culture seedlings ——


Therefore, strictly controlling the air cleanliness of the plant tissue culture room and creating sterile inoculation rooms and sterile culture rooms are the only ways for plant factories to reduce the pollution rate of tissue culture seedlings and increase the success rate of seed culture.


TWO  KLC tissue culture room clean air clean program


As an environmental technology company that has been in the field of air purification for many years, KLC has provided comprehensive and effective clean air solutions and air purification products for many large domestic plant factories, from the general environment of the tissue culture room to the local environment of the inoculation operation. Strictly control the air cleanliness, not letting go of any dust particles.


01 KLC clean bench series

As the core purification equipment for tissue culture and inoculation, the clean bench can provide a local dust-free and sterile working environment to ensure that the operating area reaches a hundred-level cleanliness.

The clean bench series developed by KLC are all equipped with KLC non-partitioned high efficiency filters, with a filtration efficiency of 99.999%@0.3μm, and include two air supply modes, horizontal flow and vertical flow. Large operating space, low vibration and low noise provide a comfortable, safe and pollution-free operating environment for the inoculation staff.


image.pngApplication example


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▲ Horizontal flow clean bench customized by KLC according to customer needs.


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▲ The KLC square horizontal flow table makes the inoculation operation area reach a hundred-level cleanliness.


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▲ KLC double horizontal flow table, effectively saving space.


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▲ Every KLC clean workbench adopts KLC's own high efficiency filter without partitions, 

strictly controls every link of production, and provides the most powerful guarantee for product quality.


02 KLC Pass Box series

The Pass Box is an auxiliary device for transferring small objects between areas of different cleanliness levels in the plant tissue culture room.

The specialized Pass Box of KLC in tissue training room is made of high-quality anti-corrosion and corrosion-resistant sheet material, which is resistant to high humidity and corrosion; it has ultraviolet sterilization and electronic interlocking functions, which can effectively reduce the number of door openings in the clean room and reduce the probability of pollution transmission to a minimum.


image.pngApplication example


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▲ The KLC Pass Box connecting the sterilization room and the inoculation room effectively reduces the chance of medium contamination


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▲ KLC Pass Box is made of high-quality stainless steel sheet, which is rust and corrosion resistant.


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▲ KLC can customize Pass Box of different specifications and materials according to the 

different needs of customers to adapt to different cleanliness, temperature/humidity environments.


03 KLC Air/Cargo shower room series

The Air/Cargo shower room is a passage that people/goods must pass through in the plant tissue culture room. The clean strong wind can take away the dust attached to the surface of people or objects, which can well prevent the operator/goods from entering and leaving the tissue training room. The pollution problem caused.

KLC can provide customers in plant factories and flower and seedling planting industries with a variety of wind/cargo shower products suitable for different scenarios. With excellent dust removal effect and durability, it strictly guards the first checkpoint of people/goods in and out.


image.pngApplication example


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▲ KLC customized stainless steel air shower room according to customer needs, with stainless steel nozzles, 

which can quickly blow out clean air and take away dust particles from personnel.


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▲ KLC common air shower room connecting the sterile room and the dressing room.


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▲ After changing the dust-free clothes, the staff must be thoroughly blown before entering the sterile room.


In addition to the core purification equipment of the plant tissue culture room, KLC can also provide customers with the primary, middle and high efficiency air filters required for the air purification system of the tissue culture room, which circulates the air and establishes a positive pressure environment to avoid external air pollution and ensure the establishment Dust-free and sterile environment.


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▲ The air cleanliness level of the inoculation room in the tissue training room is recommended to be Class 10,000, and the operating area of the clean workbench reaches the local Class 100. Illumination is greater than 300LUX; adjacent area of pressure difference 5Pa.


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▲ The recommended air cleanliness level of the culture room in the tissue culture room is 100,000, and the temperature is maintained at 20~27℃.



THREE  KLC cooperative customers


KLC has so far provided a full range of clean air solutions and air purification products for many well-known large-scale flower and seedling planting enterprises in China. We have won the recognition of many customers with our high-quality products and considerate service. (List of some customers)


Huitong Agriculture

Founded in 2008, it is an agricultural modernization company specializing in agricultural scientific research and development, tissue cultivation, seedling cultivation, semi-finished products and finished products maintenance, and then sales. The cultivation bases are all over the Pearl River Delta region: Panyu, Nansha, Nanhai, Dongguan, etc. The annual production capacity reaches more than 60 million plants, and the products are exported to all parts of the country, and some varieties are exported to Asia-Pacific and European and American countries. The production and sales are developing rapidly at a rate of 30% every year. 


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Colorful gardening

Established in Hong Kong in 1979, it is China's flower gardening company with the longest history, the deepest accumulation, and the largest variety of flowers. The company builds flower farms according to the different climate needs of different flowers. It has established more than 20 production bases in Guangdong, Yunnan, Hainan and other places with an area of nearly 5,000 acres. In addition, it has branches in Beijing, Shanghai, Sichuan, the Netherlands, and Japan. The company or its branches have established domestic advanced tissue training rooms in Guangdong and Yunnan at the same time, with nearly 200 independent accounting and independent business units, with nearly 2,000 employees, and it is still expanding.


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Golden Grass Bio

Established in 2010, it is a wholly-owned subsidiary of Fujian Jincao Biological Group Co., Ltd. Relying on technical forces such as the School of Life Sciences of Xiamen University, the School of Medicine, and the Fujian Forestry Science and Technology Experiment Center, the company has successfully developed the tissue culture seedlings of the rare medicinal plants of Anoectochilus, and has mastered the cultivation techniques in different seasons under the existing climatic conditions , Its tissue culture and rapid propagation technology can be directly applied to factory seedlings.


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With the continuous development of plant tissue culture technology, this technology will show greater potential in genetic breeding and variety breeding. Plant factory breeding and seedlings will move towards modernization, large-scale, and automation. How to effectively reduce the pollution rate of artificial inoculation and cultivation, and improve the survival rate and efficiency of tissue culture seedlings? I believe KLC can help you.


Some pictures are from the Internet, delete if invaded

For more information about purification products, please contact KLC sales staff or visit KLC official website for more information.


KLC has nearly 20 years of experience in the purification industry. Since its establishment, it has been committed to product development and development in the field of air cleaning. It has been driven by innovation to continuously improve its products and services. KLC has provided advanced, high-quality, comprehensive clean air solutions and perfect technical services for various fields and industries at home and abroad, so that you can experience safety, reliability and confidence in the cooperation with KLC.